WebCD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic ... WebImaging with Lectin Fluorescent Conjugates. Fluorescent lectins have been used to localize and detect cell-surface or intracellular glycoconjugates, making them versatile primary detection reagents for …
Pierce™ NHS-Fluorescein Antibody Labeling Kit
Web4. For 2.5 mg of antibody or other protein, pipette 500 µl of FITC mixture into the antibody solution and vortex immediately. This provides a conjugation ratio of 200 µg FITC to 1 mg antibody/protein. Mix end-over-end for 2 hours at room temperature, or transfer to a flask and mix with a stir bar and magnetic stirrer. WebIHC-Fr (1:10-1:200); Flow Cyt (1:1000). The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. rbc heritage golf tournament 2021 winner
Anti-FITC MicroBeads Any cell type MicroBeads and Isolation …
WebWe recommend FITC Conjugation Kit ab188285 as an alternative this kit using a faster, newer protocol. To conjugate an antibody to FITC using this kit: - add modifier to … WebPurification is quickly achieved using the pre-packed spin column supplied with the kit, resulting in >85% recovery of antibody conjugate. The entire labeling and purification procedure can be completed in as little as 90 minutes. Removal of free dye is essential for the determination of the degree of labeling (DOL). WebFLUORESCEIN (FITC) CONJUGATION PROTOCOL This protocol describes the conjugation reaction for 0.5 mL of antibody (IgG) at a concentration of 0.5 mg/mL (0.25 mg, corresponding to 1.67 nmol IgG). This protocol uses a 60X molar excess of FITC to generate IgG-FITC conjugates with estimated F/P ratios of 4-7. Other volumes and rbc heritage golf tournament 2021 results