WebWe detail here a protocol using tandem-tagged mCherry-EGFP-LC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations of cells based on ... WebOct 11, 2024 · Cells were photographed using an Eclipse 50i fluorescent microscope (Nikon Instruments, Tokyo, Japan) with an Olympus DP70 camera (Tokyo, Japan). Photomicrographs of multiple fields were taken using blue (to detect nuclei), red (to detect mCherry), and green (to detect FITC) filters.

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WebFluorophore Selection. Fluorescence SpectraViewer. Our labeling reagents enable researchers to create their own labeled biomolecule for use in immunochemistry, fluorescence in situ hybridization (FISH), cell tracing, receptor labeling, and cytochemistry applications as well as for probing biological structure, function, and interactions. WebmCherry Antibody (1C51) [FITC] Summary. This mCherry Antibody (1C51) was developed against recombinant full-length mCherry purified from E. coli. This mCherry Antibody … iowa allergy clinic des moines

Which fluorophores are useful for flow cytometry - Bio-Rad

WebThe red mFruit proteins, mApple, mCherry and mStrawberry (emission peaks at 592, 610, and 596 nanometers, respectively), have brightness levels ranging from 50 percent to 110 percent of EGFP, but mApple and mCherry are far more photostable than mStrawberry and are the best probe choices to replace mRFP1 for long-term imaging experiments ... WebCytoFlex LX A and B. Both CytoFLEX analyzers are a five laser system capable of detecting in 19 different channels (please see the configuration below for details), with a slight … WebJan 1, 2024 · The C-peptide-mCherry-hiPSC line (HMGUi001-A-8) was generated by heterozygous insertion of the mCherry sequence at the C-terminus of the C-peptide, which is encoded by exon 3 of the insulin locus (Table 1, Fig. 1 A).The inserted mCherry sequence was followed by a repeat of the last two amino acids of C-peptide, as they are … iowa allergy asthma \u0026 immunology pc