Germacradienol/geosmin synthase
WebDec 15, 2011 · In this study, we report new putative geoA and tpc sequences, encoding germacradienol/geosmin synthase and 2-methylisoborneol synthase, respectively, from different Streptomyces strains. Presence of geoA -related sequences correlated with the detection of geosmin in culture of these strains, suggesting that these sequences … WebNov 1, 2008 · Geosmin synthase(GS)-catalyzed cyclization of farnesyl diphosphate (FPP) to geosmin, germacradienol, germacrene D, and 8,10-dimethyl-1-octalin. Germacradienol and germacrene D are formed by the N-terminal domain of the bifunctional protein, and geosmin is generated from germacradienol via the octalin by the C-terminal domain.
Germacradienol/geosmin synthase
Did you know?
WebThe biosynthesis pathway of geosmin has been well char - acterized in bacteria, particularly in the actinobacterial genus Streptomyces. A bi-functional enzyme, germacradienol/ geosmin synthase, has been identied to be specic for bio - synthesis of geosmin. The N-terminal domain of the pro-tein converts farnesyl diphosphate (FPP), the immediate WebSep 25, 2015 · Geosmin synthase from Streptomyces coelicolor N-terminal domain complexed with three Mg2+ ions and alendronic acid. ... Germacradienol/geosmin …
http://www.reeis.usda.gov/web/crisprojectpages/0233665-relationship-of-geosmin-biosynthesis-to-plant-growth-crop-production-and-food-quality-in-beta-vulgaris.html WebApr 1, 1999 · The enzyme mediates a key step in the biosynthesis of geosmin (see EC 4.1.99.16), a widely occurring metabolite of many streptomycetes, bacteria and fungi. Also catalyzes the reaction of EC 4.2.3.75 .
WebJun 1, 2006 · Incubation of recombinant germacradienol synthase, encoded by the SCO6073 (SC9B1.20) gene of the Gram-positive soil bacterium Streptomyces coelicolor, … WebApr 1, 1999 · germacradienol synthase Alternative Name(s) germacradienol/germacrene-D synthase: Reaction catalysed (2E,6E)-farnesyl diphosphate + H2O => (1E,4S,5E,7R) …
WebJan 29, 2003 · Expression and mechanistic analysis of a germacradienol synthase from Streptomyces coelicolor implicated in geosmin biosynthesis PNAS Biochemistry …
WebApr 21, 2024 · Incubation of this germacradienol/geosmin synthase with [1,1-(2)H2] FPP (2a) gave geosmin-d1 (1a), as predicted. When recombinant GeoA from either S. avermitilis or S. coelicolor A3(2) was ... ghost by raina telgemeier summaryWebdiphosphate (FPP) to geosmin via germacradienol (Fig.1) and elucidated one of the terpene metabolite pathways in Streptomyces [16, 17]. We recently isolated a sesquiterpene synthase named spterp13 from Streptomyces peucetius ATCC 27952; this was characterized as a germacradienol synthase that catalyzed the Mg2+-dependent … ghost by ryan caraveoGeosmin synthase or germacradienol-geosmin synthase designates a class of bifunctional enzymes (EC 4.1.99.16) that catalyze the conversion of farnesyl diphosphate (FPP) to geosmin, a volatile organic compound known for its earthy smell. The N-terminal half of the protein catalyzes the conversion of farnesyl diphosphate to germacradienol and germacrene D, followed by the C-terminal-mediated … frontbordWebGeosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase ... front book vs back bookWebAug 15, 2024 · The geosmin synthase of Streptomyces coelicolor A3(2) is a Mg 2+-dependent bifunctional enzyme. The N-terminal domain of the protein converts FPP to germacradienol, while the C-terminal domain catalyzes the transformation of germacradienol to geosmin (Jiang et al. 2006; Jiang and Cane 2008). ghost by raina telgemeier pdfWebApr 6, 2024 · The enzyme geosmin synthase catalyses the cyclization of farnesyl diphosphate to germacradienol and germacrene D, and then converts germacradienol to geosmin 4. front bootstrapWebgermacradienol seems to be the committed step in the formation of geosmin, the characteristic odoriferous constituent of Strepto-myces species. secondary … ghost by raina telgemeier quotes